Fig 3. HopZ3 acetylates SlRIN4-1,-2, -3, AvrPto1Psy, PTO, SlRIPK but not FEN or AvrPtoBPsy.
Purified recombinant His-tagged SlRIN4-1, -2, -3, AvrPto1Psy, AvrPtoBPsy and GST-tagged PTO, FEN and SlRIPK proteins were incubated with His-tagged HopZ3 or HopZ3_C300A mutant (C/A) in the presence of IP6 and 14C-acetyl-CoA for 2 h at 30°C. Samples were separated by SDS-PAGE and subjected to autoradiography for 14 days. (A) SlRIN4-1, -2, -3 and AvrPto1Psy were acetylated by HopZ3. (B) PTO and SlRIPK were acetylated by HopZ3; however, FEN acetylation was not detected. (C) AvrPtoBPsy was not significantly acetylated by HopZ3.