Table 1. HopZ3 interacts with members of a tomato immune complex.
| Yeast two-hybrid | In vitro pull-down | BIFC in N. benthamiana | ||||
|---|---|---|---|---|---|---|
| HopZ3 | AvrPto1Psy | HopZ3 | AvrPto1Psy | HopZ3 | AvrPto1Psy | |
| SlPTO | - | + | + | + | + | weak |
| SlFEN | weak | + | + | - | weak | - |
| SlRIN4-1 | + | weak | + | -/weak | + | + |
| SlRIN4-2 | + | weak | + | weak | + | + |
| SlRIN4-3 | nd | nd | + | - | + | + |
| SlRIPK | weak | + | nd | nd | + | + |
| AvrPto1Psy | + | + | + | nd | + | weak |
| AvrPtoBPsy | + | + | + | + | + | -/weak |
Interacting partners of HopZ3 and AvrPto1Psy in yeast two-hybrid analysis, in vitro pull-down and in planta BIFC are shown. + indicates interaction; weak indicates weak signal; -, no interaction (no signal); nd, not determined. When interactions were tested in two directions, the stronger score is reported in the table. See also Figs 2 and S1, S2 and S3 for details of interactions in different tests and additional combinations.