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. 2021 Nov 11;16(11):e0259809. doi: 10.1371/journal.pone.0259809

Table 1. Purification of recombinant HypZn.

Purification steps Total protein (mg) Total activity (U) Specific activity (U/mg) Purification fold Recovery rate (%)
Crude extract 80.0 97868.3 1223.4 1 100
85% Ammonium sulfate precipitation 64.2 89857.9 1399.7 1.1 80.0
Sephacryl S-200 chromatography 42.3 78644.5 1859.2 1.5 52.9