Table 1. Purification of recombinant HypZn.
Purification steps | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Purification fold | Recovery rate (%) |
---|---|---|---|---|---|
Crude extract | 80.0 | 97868.3 | 1223.4 | 1 | 100 |
85% Ammonium sulfate precipitation | 64.2 | 89857.9 | 1399.7 | 1.1 | 80.0 |
Sephacryl S-200 chromatography | 42.3 | 78644.5 | 1859.2 | 1.5 | 52.9 |