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. 2021 Nov 5;22(21):11995. doi: 10.3390/ijms222111995

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Neuroregenerative capability by the combination treatment hUCB and rhEPO group was increased in the HIE model through a synergistic effect. (A) Up-regulation of the levels of Nestin, Sox2, and pro-BDNF (32 kDa) protein was shown by Western blot. (B–D) The graphs depict band intensity. (E) Neuroprotection was observed through immunohistochemistry staining for NeuN in the peri-infarct cortex of the HIE brain. White bar indicates 100 µm. (F) The graph depicts the quantification of the number of NeuN (+cells. Data shown as the mean ± standard error of mean. n = 3 per group. * p < 0.05 ((B,D) Kruskal–Wallis; (C,F) one-way ANOVA).