Figure 8.

Schematic timeline for assessment of the effects of hUCB and rhEPO in ameliorating mouse brain lesions and behavioral outcomes. Postnatal day 7 (P7) mice were induced with a hypoxic–ischemic brain injury. On P14, the hUCB (3 × 10⁷/kg) was injected, administered via intraperitoneal injection, and rhEPO (500 IU/kg) was given intraperitoneally from the beginning on P14 for five consecutive days (white arrows). MK-2206 (100 mg/kg) was given intraperitoneally from the beginning on P14 for five consecutive days from 1 h before hUCB and rhEPO treatment. One week after injection, the mice were sacrificed for qRT-PCR and Western blot (red arrows). For long-term follow-up, the mice were assessed for behavioral test and histologic experiments at P42 (red arrows).