Figure 7.

Clioquinol (CQ) suppresses the accumulation of sarkosyl-insoluble tau in M1C cells. Fractionational analysis was performed following CQ treatment, and cells were separated into SN1 (Tris soluble), SN2 (Tris insoluble-sarkosyl-soluble), and S/P (sarkosyl-insoluble pellet) fractions. Each fraction was then analyzed by Western blot using Tau5 (A), and PS199/202 antibodies (B). The amount of tau was decreased in S/P fractions obtained from cells treated with CQ. The ratio of phosphorylated tau vs tau detected by non-phospho dependent antibody Tau5 by 5 μM CQ also decreased, which was detected by antibodies against PS199/202. 0: 0 μM CQ, 5: 5 μM CQ. n = 4, * p < 0.05, ** p < 0.01, NS: not significant, Bar ± SD. All the data followed a normal distribution and were analyzed by the Student’s t-test.