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. 2021 Nov 2;22(21):11906. doi: 10.3390/ijms222111906

Figure 5.

Figure 5

Rescue experiments using T3 in shDIO2-cardiomyocytes. (A) Effects of DIO2 knockdown (n = 7 independent samples) and T3 (10 nM) rescue (n = 3 independent samples) in shDIO2-CMs on basal respiration, proton leak, maximal respiration, respiratory reserve, non-mitochondrial oxygen consumption, ATP-linked respiration, coupling efficiency (%) and spare respiratory capacity (%). The dashed lines represent the relative expression levels of shSCR-CMs; * p < 0.05; Student unpaired t-test; values are compared to the mean shSCR-CM level. (B) Cellular ROS levels in shSCR- and shDIO2-CMs under control conditions and with the addition of T3 (10 nM) as a rescue experiment (n = 8 independent samples. (C) RT-qPCR derived expression profiles of cardiac metabolic genes (PDK4, PGC1α, GDF15, and PPARα; n = 4 independent samples; corrected for 36B4) in rescue experiments with and without the addition of T3 (10 nM); ● = shSCR Ctrl; ■ = shSCR + T3; ▲ = shDIO2 Ctrl; ▼ = shDIO2 + T3. (D) Immunofluorescence staining of Ki-67 indicating proliferative activity in percentage positive cells ((n = 5 independent samples). * p < 0.05; Student unpaired t-test; values are compared to the mean shSCR-CM level. Data shown are expressed as means ± standard error of the mean (SEM).