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. 2021 Nov 1;17(11):e1010020. doi: 10.1371/journal.ppat.1010020

Fig 5. The rv0180c gene allows invasion of hMDMs via CR3.

Fig 5

(A) hMDMs were pretreated for 30 min with 10 μg.ml-1 mAb directed against the I-domain (clone 2LPM19c) or the lectin-site (clone VIM12) of human CR3, or normal mouse IgG1 used as control or (B) hMDMs were transfected with non-targeting control siRNA or CD11b-targeting siRNA. Insert showed cytofluorimetric analysis of membrane expression of CD11b from a representative CD11b silencing experiment in hMDMs. (A,B) hMDMs were then incubated with either the parental H37Rv, the Δrv0180c::km mutant, or the Δrv0180c::km complemented strains at MOI 10:1 for 60 min at 37°C, fixed and prepared for analysis by confocal fluorescence microscopy. Vertical bar plots represented the percentage of infected cells. The values are means +/- SEM calculated from 4 (A) and 3 (B) donors. The significance of difference between strains was evaluated using the one-way ANOVA followed by Bonferroni’s post hoc test. The significance of difference between control and treatment was determined using the paired Student’s t-test; *p<0,05, ** p<0,01.