Skip to main content
. 2021 Nov 1;17(11):e1010020. doi: 10.1371/journal.ppat.1010020

Fig 6. Removal of the bacterial capsule suppresses the differential uptake of the Δrv0180c::km mutant.

Fig 6

The parental H37Rv, the Δrv0180c::km mutant, or the Δrv0180c::km complemented strains were grown (A) in 7H9 ADC liquid medium with or without Tween-80 0.05% or (B) in Sauton liquid medium before removal of the capsule using agitation with glass beads. Untreated bacteria were used as control. (A,B) hMDMs were then incubated with bacterial preparations at MOI 10:1 for 60 min at 37°C, fixed and processed for analysis by confocal fluorescence microscopy. Vertical bar plots represented the percentage of infected cells. The values are means +/- SEM calculated from 3 donors. The significance of difference between strains was evaluated using the one-way ANOVA followed by Bonferroni’s post hoc test; *p<0,05, ** p<0,01.