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. 2021 Nov 4;22(21):11949. doi: 10.3390/ijms222111949

Figure 5.

Figure 5

High-speed cosedimentation experiments of MyBPC C0–C2 with F-actin and β-cardiac myosin. (A) Actin (0–40 µM) and MyBPC C0–C2 (5 µM) were cosedimented and the fraction of C0–C2 in the pellet was determined. A quadratic fit yields the dissociation constants Kd for wt of 3.2 ± 0.9 µM and D389V of 2.8 ± 0.6 µM. (B) MyBPC C0–C2 (0–30 µM) was cosedimented with 2.5 µM of β-cardiac myosin. The amount of C0–C2 bound to synthetic thick filaments was determined using a protein standard range of C0–C2. A quadratic fit yields Kd values for wt of 12.7 ± 4.7 µM and D389V of 14.8 ± 7.7 µM. Data points indicate mean ± SD from at least n = 3 experiments.