β-CM	β-Cardiac myosin
CD	Circular dichroism
cvSMD	Constant velocity steered molecular dynamics
DSC	Differential scanning calorimetry
DTT	Dithiothreitol
GBSA	Generalized Born and surface area continuum solvation
IPTG	Isopropyl β-d-1-thiogalactopyranoside
MyBPC	Cardiac myosin-binding protein C
PBS	Phosphate buffered saline
SDS-PAGE	Sodium dodecyl sulfate–polyacrylamide gel electrophoresis
SMD	Steered molecular dynamics
TAME	1,1,1-Tris(aminomethyl)ethane
Tm	Protein melting temperature
TPCK	Tosyl phenylalanyl chloromethyl ketone
TSA	Thermal shift assay
UPS	Ubiquitin–proteasome system
vdW	Van der Waals
Wt	Wild type