Table 1.
Structural and thermodynamic analysis of N-terminal MyBPC domains as determined by CD experiments, TSA and DSC. α: α-helix, β: β-sheet, T: turn, U: unordered, Σ: total, Tm: melting temperature. Data are mean ± SEM of at least n = 3 experiments.
| Construct | α (%) | β (%) | T (%) | U (%) | Σ (%) | Tm (TSA)/°C | Tm (DSC)/°C |
|---|---|---|---|---|---|---|---|
| C2 wt | 7 | 37 | 22 | 34 | 100 | 59.3 ± 0.1 | 56.5 ± 0.3 |
| C2 D389V | 7 | 36 | 22 | 35 | 100 | 52.1 ± 0.3 | 52.3 ± 0.2 |
| C0–C2 wt | 7 | 36 | 24 | 32 | 99 | 51.8 ± 0.5 | 50.2 ± 0.1 |
| C0–C2 D389V | 8 | 40 | 22 | 30 | 100 | 48.9 ± 0.6 | 47.6 ± 0.3 |