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Reduction of SELENOF increases oxygen consumption in RWPE-1 cells. Average fold changes are represented for (A) basal, (B) maximal respiration after FCCP injection, (C) spare respiratory capacity (difference in peak OCR and basal measurements), and (D) mitochondrial ATP production (difference before and after oligomycin injection). Data are presented as the mean ± SEM, n =3, two-tailed paired t-test, * p < 0.05, ** p < 0.01, n = 3.
