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HPLC assays displaying that the best peptide from the bioinformatic screen (CSLMQ) was able to show near-identical conversion to a native tetrapeptide sequence CVLS (85% vs. 84%, respectively). The quantification was performed as in Figure 4. Reactions contained 2.4 µM peptide, 10 µM FPP, and 25 nM enzyme. (A) DsGRAGCSLMQ with 25 nM rFTase; (B) DsGCVLS with 25 nM yFTase.
