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. 2021 Oct 20;10(21):4801. doi: 10.3390/jcm10214801

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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IFN-γ levels at 1 and 3 days after laser-induced ocular hypertension (OHT). (a) The IFN-γ values obtained in the multiplex assay. The histogram shows the mean levels (±SD) of IFN-γ (pg/mg) at days 1 and 3 after laser OHT induction in saffron ocular hypertension eyes (saffron OHT), saffron-contralateral eyes (saffron-contralateral), and saffron-naïve eyes. (b) Immunohistochemical study of IFN-γ expression in saffron-contralateral eyes three days after unilateral laser-induced OHT. Retinal sections were immunolabeled with antibodies to IFN-γ (green) and Iba-1 (red). Merge is denoted with the colour yellow. The arrowhead shows the co-expression of Iba-1 and IFN-γ in saffron-contralateral and saffron-naïve eyes. Abbreviations: OHT (ocular hypertension); IFN-γ (interferon-γ); Iba-1 (ionized calcium-binding adaptor molecule).