Figure 7.

IL-4 levels at 1 and 3 days after laser-induced ocular hypertension (OHT). (a) The IL-4 obtained in the multiplex assay. The histogram shows the mean levels (±SD) of IL-4 (pg/mg) at days 1 and 3 after laser OHT induction in saffron ocular hypertension eyes (saffron OHT) and saffron-contralateral eyes (saffron-contralateral) as well as in naïve eyes. (b) Immunohistochemical study of IL-4 expression in saffron-contralateral eyes three days after unilateral laser-induced OHT. Retinal sections were immunolabeled with antibodies to IL-4 (green) and Iba-1 (red). Merge is denoted by the colour yellow. The arrowhead shows the co-expression of Iba-1 and IL-4 in saffron-contralateral and saffron-naïve eyes. Abbreviations: OHT (ocular hypertension); IL-4 (interleukin- 4); Iba-1 (ionized calcium-binding adaptor molecule).