Table 1.
Antibodies employed for the immunostaining analysis and their corresponding concentrations.
| Colour | Primary Antibody | Conc. | Secondary Antibody | Conc. |
|---|---|---|---|---|
| GREEN | Rabbit polyclonal anti-IL-1β (ref. ab9722, Abcam plc) [44] | 1:250 | Goat anti-rabbit Alexa Fluor 488 (ref. ab150077, Abcam plc) |
1:150 |
| Rabbit polyclonal anti-IL-6 (ref. ab208113, Abcam plc) [45] | 1:200 | |||
| Rabbit polyclonal anti-IL-17 (ref. ab79056, Abcam plc) [46] | 1:300 | |||
| Rabbit polyclonal anti-IFNγ (ref. ab9657, Abcam plc) [47] | 1:300 | |||
| Rabbit monoclonal anti-BDNF (ref. ab213323, Abcam plc) [44] | 1:250 | |||
| Rabbit monoclonal anti-VEGF receptor 1 (ref. ab32152, Abcam plc) [48] | 1:200 | |||
| Rabbit polyclonal anti-CX3CL1 (ref. ab25088, Abcam plc) [49] | 1:500 | |||
| Rabbit polyclonal anti-TNFα (ref. ab9739, Abcam plc) [50] | 1:300 | |||
| Rat monoclonal anti-IL-4 (ref. ab11524, Abcam plc) [51] | 1:250 | Goat anti-rat Alexa Fluor 488 (ref. ab150165, Abcam plc) |
1:150 | |
| Rat monoclonal anti-IL-10 (ref. ab189392, Abcam plc) [52] | 1:200 | |||
| RED | Rabbit polyclonal anti-Iba1 Red Fluorochrome 635 conjugated (ref. 5100756, Wako Chemicals GmbH) [53] | 1:200 | ||
| Chicken polyclonal anti-GFAP (ref. AB5541, Sigma-Aldrich) [54] | 1:200 | Goat anti-chicken IgY (H+L) Alexa Fluor 594 (ref. A-11042, Invitrogen) | 1:300 | |
| Rabbit polyclonal anti-NF-200 (ref. N4142, Sigma-Aldrich) [55] | 1:150 | Donkey anti-rabbit IgG1 Alexa Fluor 594 (ref. A21207, Invitrogen) | 1:800 |
Antibodies used in the study: interleukin 1 beta (IL-1β), interleukin 6 (IL-6), interleukin 17 (IL-17), interferon gamma (IFN-γ), brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF), fractalkine (CX3CL1), tumour necrosis factor alpha (TNF-α), interleukin 4 (IL-4), and interleukin 10 (IL-10); also included are the antibodies used to identify microglial cells (Iba-1), axons (NF-200 KDa), and macroglia (GFAP). The colour (green/red) indicates how the immunostaining is labelled.