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. 2021 Nov 8;22(21):12079. doi: 10.3390/ijms222112079

Figure 2.

Figure 2

ALDH1B1 and AMBRA1 inversely regulate PTEN transcription. (A) Schematic flow diagram of the enzyme activity of ALDH1B1 regulating the transcription of PTEN. (B) mRNA levels of ALDH1B1 and PTEN in HCT116 cell lines stably expressing Myc-ALDH1B1, as assessed by RT-qPCR (n = 4). (C) mRNA levels of ALDH1B1 and PTEN in HCT116 cells overexpressing the indicated amounts of 3×M vector (pCMV-3Tag-2, empty vector) and Myc-ALDH1B1, as assessed by RT-qPCR (n = 3). (D) mRNA levels of ALDH1B1 and PTEN in control (shLUC) and ALDH1B1-depleted (shALDH1B1 #1, #2) HCT116 cells, as assessed by RT-qPCR (n = 3). Symbols: shLUC KD, control knockdown; ALDH1B1 CDS KD, knockdown targeting the coding region of ALDH1B1; 3’ UTR KD, knockdown targeting 3’ UTR region. (E) mRNA levels of AMBRA1 and PTEN in HCT116 cells transiently overexpressing empty vector or the indicated amounts of Flag-AMBRA1, as assessed by RT-qPCR (n = 3). (F) mRNA levels of AMBRA1 and PTEN in AMBRA1-deficient HCT116 cells, as assessed by RT-qPCR (n = 3). In (B–F), mRNA levels were normalized to that of GAPDH and the expression levels in the control were set at 1. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control.