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. 2021 Nov 8;22(21):12079. doi: 10.3390/ijms222112079

Figure 3.

Figure 3

AMBRA1 affects the mRNA levels of PTEN, CTNNB1 (β-catenin), and CSC-associated β-catenin target genes in an ALDH1B1-dependent manner. (A) mRNA levels of PTEN in wild-type (WT) and ALDH1B1-knockdown HCT116 cells overexpressing empty vector or the indicated amounts of Flag-AMBRA1, as assessed by RT-qPCR (n = 3). (B) mRNA expression levels of CTNNB1 in control (shLUC) and ALDH1B1-depleted (shALDH1B1 #1, #2) HCT116 cells overexpressing empty vector or the indicated amounts of Flag-AMBRA1, as assessed by RT-qPCR (n = 3). (C) mRNA expression levels of β-catenin target genes associated with cancer stem cells (CSCs), as assessed by RT-qPCR using the same samples as those used in (B) (n = 3). In (A–C), mRNA expression levels were normalized to that of GAPDH and the mRNA levels in the control were set at 1. In WT HCT116 cells, mRNA levels after AMBRA1 overexpression were compared with the control. The average mRNA level after overexpressing AMBRA1 at the two indicated amounts in ALDH1B1-depleted HCT116 cells was compared with that in WT HCT116 cells. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control.