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. 2021 Nov 8;22(21):12085. doi: 10.3390/ijms222112085

Figure 4.

Figure 4

Dunnione attenuates breast cancer-associated lung thrombosis via the NAD(P)H quinone oxidoreductase 1 (NQO1) −nicotinamide adenine dinucleotide (NAD) −sirtuin 1 (SIRT1) −nuclear factor−kappa B (NF-kB) −tissue factor axis in vivo. (A) Representative IHC images for tissue factor staining of the lung sections in each group. Scale bar, 100 μm. (B,D) Western blotting analysis of acetyl-NF-κB, tissue factor (B), and SIRT1 (D) expression in lung tissues (n = 4 for SIRT1, others 5 per group). Densitometric analysis is presented as the fold induction of the target protein relative to β-actin. (C) NAD+ levels in lung tissues were assessed by using the NAD+/NADH assay kit in the mice of each group (n = 5 per group). (E) SIRT1 activity in lung tissues was measured using the SIRT1 activity assay kit (n = 4 per group). Data represent the mean ± SD. p-values were obtained by one-way ANOVA. Significance as compared to the samples of the 4T1 group. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.