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. 2021 Nov 2;22(21):11895. doi: 10.3390/ijms222111895

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characterisation of plastoglobules (PGs) from pea and bean plants: (A) localisation of PGs (green) in chloroplasts (red) observed with a CLSM (Confocal Laser Scanning Microscope), and topology of PGs in control conditions (yellow-to-white colouring) and after seventh day of low-temperature treatment (blue-to-white colouring) using AFM techniques; (B) elasticity of isolated PGs measured via Young’s modulus using AFM from mature plants in control conditions (Pea, Bean), after seven days of low-temperature treatment (ch), and in first leaves (fl). Presented data are mean values ± SD from three independent experiments; pairs of results marked with an asterisk differ significantly at p = 0.05 (one-way ANOVA with posthoc Tukey test).