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A clot waveform analysis for thrombin time. (I) Calibration plasma; (II) FVIII-deficient plasma; (a) thrombin 0.01 IU/mL; (b) thrombin 0.05 IU/mL; (c) thrombin 0.1 IU/mL; (d) thrombin 0.5 IU/mL; (e) thrombin 1.0 IU/mL; (f) thrombin 5.0 IU/mL. Navy line, fibrin formation curve; red line, 1st derivative curve (velocity); light blue, 2nd derivative curve (acceleration).
