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. 2021 Nov 8;22(21):12074. doi: 10.3390/ijms222112074

Figure 1.

Figure 1

The effect of Pulmozyme® and DNase I on the viability, apoptosis and migration of melanoma B16 cells. (A) Viability of B16 melanoma cells in presence of Pulmozyme® or DNase I ((0.1–0.75) × 103 U/mL) for 48 h. Number of living cells in the control (cells incubated in absence of enzyme) was taken as 100%. Data are presented as mean ± SEM. (B) Apoptosis of B16 cells after treatment with Pulmozyme® and DNase I (0.5 × 103 U/mL). Flow cytometry analysis of B16 cells stained with Annexin V-FITC/PI 24 h after treatment is shown. Q3-1, Annexin V-FITC−/PI+, necrosis; Q3-2, Annexin V-FITC+/PI+, late apoptosis; Q3-3, Annexin V-FITC−/PI− cells; Q3-4, Annexin V-FITC+/PI− early apoptosis. (C) Photographs of scratch healing by B16 cells incubated with Pulmozyme® or with DNase I (0.5 × 103 U/mL) (4× magnification). Dotted line shows initial edge of the scratch at time point 0; double dotted lines show boundary of the monolayer at time point 48 h. Arrows show the movement of the migration front. (D) Dependencies of migration length of B16 cells on Pulmozyme® (red) or DNase I (blue) concentration. Data are presented for 48 h of incubation. (E,F) Extent of scratch overgrowth of B16 cells incubated with various concentrations of Pulmozyme® (D) or DNase I (E). Migration of intact cells (control)—black curve. * and **—statistically significant differences with p < 0.05 and p < 0.01.