Figure 1.

moDCs secrete IL-8, IL-10, and IL-12p70 upon treatment with vemurafenib during cytokine-induced maturation: moDCs were generated by plastic adherence, applying IL-4 and GM-CSF on days 1, 3, and 5. On day 6, cells were matured with IL-6, IL-1β, TNF, and PGE₂. Cells were additionally treated without inhibitor (no inhib), with solvent control (DMSO), vemurafenib (V), dabrafenib (D), trametinib (T), cobimetinib (C), or the clinically used combinations V + C (VC) or D + T (DT). Supernatants were sampled after 24 h. Cytokine concentrations, analyzed by Cytometric Bead Array, of eight DC batches (represented by different symbols) of six donors (DC batches of the same donor, but generated in independent experiments, have the same symbol but a different color) assessed in independent experiments, are depicted. Bars indicate mean values. p-values were determined by one-way ANOVA. In the Dunnett’s multiple comparisons test, all conditions were tested against the solvent control DMSO. *** p ≤ 0.001, **** p ≤ 0.0001, ns: p > 0.05.