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. 2021 Nov 6;22(21):12017. doi: 10.3390/ijms222112017

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Relative expressions of GmMATE1 and GmMATE2 in different tissues within the developing soybean seed pod. The relative expression of (A) GmMATE1 and (B) GmMATE2 in different tissues of the developing pod, normalized to the reference gene, VPS, using the 2^−ΔCt method. DAF, days after flowering. Values shown are the averages of three technical replicates ± SEM. Similar expression trends were obtained in another biological repeat. (C,D) Photos showing the GUS staining of (C) transgenic soybean pods and (D) transgenic A. thaliana expressing β-glucuronidase (GUS) driven by the native GmMATE1^C08 promoter. Similar results were obtained from two independent transgenic lines. Scale bar = 1 cm.