Table 4.

Oligonucleotides used for qRT-PCR. The design of oligonucleotides was carried out with the use of Primer-BLAST (www.ncbi.nlm.nih.gov) (10 August 2021). The calculated Tm for all primers is 61–63 °C. Real-time PCR was performed at Tm = 60 °C. PCR products were melted from 60 °C to 90 °C to assess the specificity of the reaction.

Genes	GenBank Accsession	Oligonucleotides 5′-3′ (F+R)	Amplicon Size, bp
Actb	NM_007393.4	CCTTCCTTCTTGGGTATGGAATCC CACCAGACAGCACTGTGTTGGCA	115
CAT	NM_009804	AGCGACCAGATGAAGCAGTG TCCGCTCTCTGTCAAAGTGTG	181
SOD1	NM_011434	AACCAGTTGTGTTGTCAGGAC CCACCATGTTTCTTAGAGTGAGG	139
NRF2	NM_010902	CTCGCTGGAAAAAGAAGTG CCGTCCAGGAGTTCAGAGG	240