Table 3. Associations between lifestyle factors and methylation level of identified CpGs, and the risk of coronary heart disease mediated through methylation level of CpG sites.
Table 3—source data 1. Association between lifestyle factors and identified CpGs.
elife-68671-table3-data1.docx (29.7KB, docx)
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Effect size(SE) | P | Mediation effect | |
|---|---|---|---|---|
| Proportion mediated, % | P | |||
| Smoking, no. of cigarettes/day |
|
|
|
|
| cg08106661 | 1.50E-04 (4.67E-05) | 0.001 | 28.50 | 0.036 |
| Diet score (ranging 0-6) |
|
|
|
|
| cg21210537 | 3.60E-03 (1.27E-03) | 0.005 | 4.66 | 0.206 |
| cg10643850 | 2.57E-03 (1.26E-03) | 0.042 | -6.91 | 0.088 |
| cg05740632 | 1.37E-03 (6.88E-04) | 0.047 | 11.30 | 0.068 |
| Body mass index, kg/m2 |
|
|
|
|
| cg20302171 | 3.90E-04 (1.67E-04) | 0.020 | -2.87 | 0.267 |
| cg08484100 | 4.17E-04 (2.10E-04) | 0.048 | -1.91 | 0.373 |
Linear regression was fitted by including all five lifestyle factors (smoking, alcohol consumption, physical activity, diet score, and body mass index) simultaneously in the same model, with methylation values as dependent variables, and age, sex, study area, fasting time, education level, marital status and batch as covariates. CpG = cytosine-phosphoguanine site; SE = standard error. Alcohol consumption and physical activity were not associated with any of the coronary heart disease-associated CpGs. Details were reported in the Table 3—source data 1.