|
Reagent type (species) or resource
|
Designation
|
Source or reference
|
Identifiers
|
Additional information
|
| HCT116 cell line (Homo-sapiens) |
Colorectal carcinoma |
ATCC |
CCL-247 |
RRID:CVCL_0291
|
| HEK-293 cell line (Homo-sapiens) |
Kidney epithelial |
ATCC |
CRL-1573 |
RRID:CVCL_0045
|
| HepG2 cell line (Homo-sapiens) |
Liver epithelial |
ATCC |
HB-8065 |
RRID:CVCL_0027
|
| MCF7 cell line (Homo-sapiens) |
Adenocarcinoma epithelial |
ATCC |
HTB-22 |
RRID:CVCL_0031
|
| PCCA mutant cell lines (Homo-sapiens) |
Propionic acidemia cell lines |
NIGMS Human Genetic Cell Repository, Coriell Institute |
GM00371 GM00405 GM22010 GM22011 GM22123 GM22126 |
|
| Transfected construct (human) |
siRNA to ACSS2 (SMARTpool) |
Dharmacon |
L-010396-00-0005 |
|
| Transfected construct (human) |
siRNA to ACLY (SMARTpool) |
Dharmacon |
L-004915-00-0005 |
|
| Transfected construct (human) |
siRNA to PCCA (SMARTpool) |
Dharmacon |
L-008965-00-0005 |
|
| Transfected construct (human) |
p300 mutant constructs |
Ortega et. al. 2018 |
WT p300, ΔGlu p300 |
|
| antibody |
ACLY, rabbit |
CST |
4,332 S |
RRID:AB_2223744, 1:1,000 dilution |
| antibody |
ACSS2, mouse |
Novus Biologicals |
NBP2-01269 |
1:1,000 dilution |
| antibody |
FFAR2/GPR43, mouse |
Novus Biologicals |
MAB10082-100 |
1:1,000 dilution |
| antibody |
FFAR3/GPR41, rabbit |
Novus Biologicals |
NBP2-14014 |
1:1,000 dilution |
| antibody |
H3, mouse |
Abcam |
ab24834 |
RRID:AB_470335, 1:5,000 dilution |
| antibody |
H3 K18ac, rabbit |
Abcam |
ab1191 |
RRID:AB_298692, 1:1,000 dilution |
| antibody |
H3 K27ac, mouse |
Active Motif |
39,685 |
RRID:AB_2793305, 1:1,000 dilution |
| antibody |
HA-Tag, rabbit |
CST |
3,724 |
RRID:AB_1549585, 1:1,000 dilution |
| antibody |
p300 (NM11), mouse |
SCBT |
sc-32244 |
RRID:AB_628076, 1:1,000 dilution |
| antibody |
p53, mouse |
CST |
2,524 |
RRID:AB_331743, 1:1,000 dilution |
| antibody |
p53 K382ac, rabbit |
CST |
2,525 |
RRID:AB_330083, 1:1,000 dilution |
| antibody |
Pan anti-acetyllysine, rabbit |
CST |
9,814 |
1:1,000 dilution |
| antibody |
Pan anti-butyryllysine, mouse |
PTM Biolabs |
PTM-329 |
1:500 dilution |
| antibody |
Pan anti-propionyllysine, rabbit |
PTM Biolabs |
PTM-201 |
1:500 dilution |
