TABLE 2.

Thirteen taxon-specific primer pairs that amplify a fragment of the 28S rDNA from Zygomycetes

PCR primer pair (5′ to 3′)	Zygomycetes species identified	Size of PCR product (bp)
Acl1 (ATCATGCGTTTGCCCTTTAGC)	Absidia coerulea	477
Acl2 (CTAAGCGAGAAAAAAGAGAAAC)
Acy1 (CGGATTGTAAACTAAAGAGCG)	Absidia corymbifera	577
Acy2 (CCAAAGTAGATTACAGTTCTAG)
Ap1 (GAATTGTAAACTTTAGAGTCGTTG)	Apophysomyces elegans	453
Ap2 (TGAACCACAGTATTTCGCGAA)
Ba1 (AAAATCTGTAAGGTTCAACCTTG)	Basidiobolus haptosporus	651
Ba2 (TGCAGGAGAAGTACATCCGC)	Basidiobolus ranarum
Cc1 (TCTCTTAACTTGCTTCTATGCC)	Conidiobolus coronatus	419
Cc2 (CTTTAATTAAGCTAATCAACATG)
Cr1 (GTGAGAATCCCGTGAATTCAC)	Cokeromyces recurvatus	434
Cr2 (CAAAGCACTCAACTATTTCGC)
Cu1 GGATTGTAAACTAAAGTTTTC	Cunninghamella bertholletiae	521
Cu2 AAATTCTCTAATTATTCCCTC	Cunninghamella elegans
	Cunninghamella polymorpha
Mc1 ATTTTCCTGGCACACCAGATT	Mucor circinelloides	538
Mc2 GCGAATAAAAAATATACTAGATGT	Mucor ramosissimus
Mp1 TGGCCGGTTTACTGGTCCGAA	Mortierella polycephala	618
Mp2 CGAGTATAAAAAGGACACGGC
Rh1 TTTTCCAGGCAAGCCGGACCG	Rhizopus azygosporus	469
Rh2 TATTCCCAGCCAACTCGCCAAAT	Rhizopus microsporus
Ro1 AGCATTTGCCTTTTGTGATACGC	Rhizopus oryzae	413
Ro2 ACCGTAGTACCTCAGAAAACC
Rm1 TCTATTGCGATGCATGCTCC	Rhizomucor miehei	305
Rm2 GGTCTCTTTAGACTCCAAAGC	Rhizomucor pusillus
Sv1 CTTTGGCTTGAGCATTGGAC	Saksenaea vasiformis	433
Sv2 AGACTAAATCAATGACTTCTGG