Figure 2.
3D immunofluorescence SIM images of A. arenosa diploid, neo-tetraploid, and established tetraploid late pachytene nuclei
(A) Mildly spread nuclei immunostained for indicated molecules to identify SCs (ZYP1), CO sites (HEI10), and axes (ASY1, where bright staining reveals asynapsis, yellow arrow, middle panel). Scale bars, 5 μm.
(B) Paths of all SCs of nuclei in (A) were computationally traced (colors arbitrary).
(C) SC paths (white) from the nuclei in (A) and (B) were segmented to identify individual bivalent and quadrivalent units, with positions of HEI10 foci (red) defined for each unit.
(D) Synaptic partner switch (SPS) for boxed quadrivalent in (C) (gold star, top), with component homologs identified (middle). Bottom: SPS is defined as a point where two SCs converge in 3D space with equal length arms.