REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rat polyclonal anti-ASY1	Armstrong et al.77	N/A
Guinea-pig polyclonal anti-ZYP1	Higgins et al.48	N/A
Rabbit polyclonal anti-HEI10	Lambing et al.78	N/A
Goat anti-rat Alexa Fluor 555 (F(ab’)2 fragment)	Abcam	Cat#: ab150162
Goat anti-guinea-pig Alexa Fluor 488	Thermofisher	RRID: AB_2534117
Goat anti-rabbit Alexa Fluor 647 (F(ab’)2 fragment)	Thermofisher	RRID: AB_2535814
Chemicals, peptides, and recombinant proteins
Colchicine	Sigma-Aldrich	Cat#: C3915
Cellulase	Sigma-Aldrich	Cat#: C1794
Pectolyase	Sigma-Aldrich	Cat#: P5936
4’,6-diamidino-2-phenylindole (DAPI)	Thermofisher	Cat#: D1306
Vectashield mounting medium	2bscientific	Cat#: H-1000-1
Cytohelicase	Sigma-Aldrich	Cat#: C8274
Polyvinylpyrrolidone	Sigma-Aldrich	Cat#: PVP40
Lipsol	Appleton Woods	Cat#: LP40023
Triton X-100	Sigma-Aldrich	Cat#: X100
Paraformaldehyde	Fisher Scientific	Cat#: 114090570
200 nm TetraSpeck microspheres	Thermofisher	Cat#: T7280
Deposited data
3D-SIM imaging data	This paper	https://doi.org/10.3929/ethz-b-000470079
Component homolog measurements	This paper	https://doi.org/10.17632/zn23pxwgz3.1
Experimental models: Organisms/strains
Arabidopsis arenosa – Streçno accession	Collected from wild populations28	N/A
Arabidopsis arenosa – Triberg accession	Collected from wild populations28	N/A
Arabidopsis arenosa – Kasparstein accession	Collected from wild populations28	N/A
Software and algorithms
Zen – Elyra PS1 2012	Zeiss	https://www.zeiss.com/microscopy/int/home.html
ImageJ – Version 2.1.0/1.53c	Schindelin et al.79	https://imagej.nih.gov/ij/
Simple Neurite Tracer Version 3.2.5	Longair et al.80	https://fiji.sc/Simple_Neurite_Tracer
MATLAB v2020b	Mathworks	R2020b
EvolvingStablePolyploidy v1.0.0 (custom MATLAB analysis code)	This paper	https://doi.org/10.5281/zenodo.5033552