Fig. 1.

Lipidomic workflow. Typical lipidomic workflows including lipid extraction, direct infusion, or separation-based mass spectrometric analysis by nominal resolution, that is, low-resolution MS or HRMS. Data analysis includes lipid identification at respective structure level, for example, lipid species or lipid molecular species level and their quantification. Important considerations to achieve high-quality results are depicted in orange and are discussed in the respective sections.