. 2021 Nov 1;48:102176. doi: 10.1016/j.redox.2021.102176

Table 2.

Fluo-5N fluorescent values corresponding to mitochondrial [Ca²⁺] before and after CAP at different extracellular [Ca²⁺]. Fluo-5N fluorescence values were normalized using Ionomycin +0 mM Ca²⁺ + EGTA (Iono EGTA) values and Ionomycin +2 mM Ca²⁺ (Iono 2 Ca²⁺) values. To determine statistical significance between NC and CAP samples at each extracellular [Ca²⁺], independent t-test were used.

Extracellular [Ca²⁺] mM		Wild-type			P-Value		CatSper KO			p-value
Extracellular [Ca²⁺] mM		Mean	SD	n	P-Value		Mean	SD	n	p-value
0	NC	0.2398	0.1556	3	0.197	NC	0.0201	0.0167	3	0.396
0	CAP	0.1935	0.1136	3	0.197	CAP	0.0234	0.0248	3	0.396
0.5	NC	0.5122	0.2798	5	0.035	NC	0.4893	0.1984	3	0.676
0.5	CAP	0.6394	0.2413	6	0.035	CAP	0.4717	0.1995	3	0.676
1	NC	0.7242	0.2798	3	0.01	NC	0.4562	0.1649	3	0.63
1	CAP	0.8402	0.208	3	0.01	CAP	0.5688	0.257	3	0.63
2	NC	0.7427	0.4494	3	0.022	NC	0.579	0.3106	3	0.155
2	CAP	1.1791	0.4635	3	0.022	CAP	0.6194	0.3805	3	0.155