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Fluo-5N fluorescence values corresponding to mitochondrial Ca2+from wild-type sperm CAP in the presence of Ru360 at different extracellular [Ca2+]. Fluo-5N values were normalized using Ionomycin +0 mM Ca2+ + EGTA (Iono EGTA) values and Ionomycin +2 mM Ca2+ (Iono 2 Ca2+) values.
| Extracellular [Ca2+] mM | Wild-type |
|||
|---|---|---|---|---|
| Mean | SD | n | ||
| 0.5 | CAP + Ru360 | 0.3483 | 0.027 | 3 |
| 1 | CAP + Ru360 | 0.5324 | 0.015 | 3 |
| 2 | CAP + Ru360 | 0.5725 | 0.0298 | 3 |
