Bleaching solution

Reagent	Final concentration	Amount
20× SSC	0.5×	1 mL
RNAse-free ddH2O	n/a	31 mL
Formamide, deionized	10%	4 mL
Hydrogen Peroxide (30%)	3%	4 mL
Total	n/a	40 mL

This solution should be prepared right before use.