REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
TotalSeq™-A0251 anti-human Hashtag 1 Antibody	BioLegend	Cat # 394601, RRID:AB_2750015
TotalSeq™-A0252 anti-human Hashtag 2 Antibody	BioLegend	Cat # 394603, RRID:AB_2750016
TotalSeq™-A0253 anti-human Hashtag 3 Antibody	BioLegend	Cat # 394605, RRID:AB_2750017
TotalSeq™-A0254 anti-human Hashtag 4 Antibody	BioLegend	Cat # 394607, RRID:AB_2750018
TotalSeq™-A0255 anti-human Hashtag 5 Antibody	BioLegend	Cat # 394609, RRID:AB_2750019
TotalSeq™-A0256 anti-human Hashtag 6 Antibody	BioLegend	Cat # 394611, RRID:AB_2750020
TotalSeq™-A0257 anti-human Hashtag 7 Antibody	BioLegend	Cat # 394613, RRID:AB_2750021
TotalSeq™-A0258 anti-human Hashtag 8 Antibody	BioLegend	Cat # 394615, RRID:AB_2750022
TotalSeq™-A0259 anti-human Hashtag 9 Antibody	BioLegend	Cat # 394617, RRID:AB_2750023
TotalSeq™-A0260 anti-human Hashtag 10 Antibody	BioLegend	Cat # 394619, RRID:AB_2750024
TotalSeq™-A0262 anti-human Hashtag 12 Antibody	BioLegend	Cat # 394623, RRID:AB_2750025
TotalSeq™-A0263 anti-human Hashtag 13 Antibody	BioLegend	Cat # 394625, RRID:AB_2750026
TotalSeq™-A0264 anti-human Hashtag 14 Antibody	BioLegend	Cat # 394627, RRID:AB_2750027
TotalSeq™-A0265 anti-human Hashtag 15 Antibody	BioLegend	Cat # 394629, RRID:AB_2750028
Mouse anti-human CD3/BUV395 (UCHT1)	BD Biosciences	Cat # 563546, RRID:AB_2744387
Mouse anti-human CD45/BUV496 (HI30)	BD Biosciences	Cat # 624283, RRID:AB_2868405
Mouse anti-human CD15/BUV563 (W6D3)	BD Biosciences	Cat # 624284, RRID:AB_2868406
Mouse anti-human CD45RA/BUV615 (HI100)	BD Biosciences	Cat # 624297, RRID:AB_2875550
Mouse anti-human CD14/BUV661 (MφP9)	BD Biosciences	Cat # 741684, RRID:AB_2868407
Mouse anti-human CD8/BUV737 (RPA-T8)	BD Biosciences	Cat # 624286, RRID:AB_2868408
Mouse anti-human CD11c/BUV805 (B-ly6)	BD Biosciences	Cat # 624287, RRID:AB_2868409
Mouse anti-human CD25/BV421 (M-A251)	BD Biosciences	Cat # 562442, RRID:AB_11154578
Mouse anti-human CD4/BV480 (SK3)	BD Biosciences	Cat # 566104, RRID:AB_2739506
Mouse anti-human CD16/BV605 (3G8)	BD Biosciences	Cat # 563172, RRID:AB_2744297
Mouse anti-human CD123/BV650 (6H6)	BioLegend	Cat # 306020, RRID:AB_2563827
Mouse anti-human CD127/BV711 (A019D5)	BioLegend	Cat # 351328, RRID:AB_2562908
Mouse anti-human IgD/BV750 (IA6-2)	BD Biosciences	Cat # 747484, RRID:AB_2868411
Mouse anti-human CD304/BV786 (U21-1283)	BD Biosciences	Cat # 743132, RRID:AB_27412
Mouse anti-human CD141/BB515 (1A4)	BD Biosciences	Cat # 565084, RRID:AB_2739058
Mouse anti-human CD11b/PerCP-Cy5.5 (M1/70)	BD Biosciences	Cat # 561114, RRID:AB_2033995
Mouse anti-human CD19/BB790 (HIB19)	BD Biosciences	Cat # 624296
Mouse anti-human CD27/PE (O323)	BioLegend	Cat # 302808, RRID:AB_314300
Mouse anti-human TCRαß/PE-Dazzle594 (IP26)	BioLegend	Cat # 306726, RRID:AB_2566599
Mouse anti-human CD34/PE-Cy5 (581)	BD Biosciences	Cat # 555823, RRID:AB_396152
Mouse anti-human CD197/PE-Cy7 (G043H7)	BioLegend	Cat # 353226, RRID:AB_11126145
Mouse anti-human CD38/APC (HB-7)	BioLegend	Cat # 356606, RRID:AB_2561902
Mouse anti-human CD56/APC-R700 (NCAM16.2)	BD Biosciences	Cat # 565139, RRID:AB_2744429
Mouse anti-human HLA-DR/APC-Cy7 (L243)	BioLegend	Cat # 307618, RRID:AB_493586
Biological samples
Cryopreserved human PBMC (longitudinal batch control)	BIOIVT	Cat # HUMAN-PBMC-U-190715
Cryopreserved human PBMC	Clinical partners	N/A
Chemicals, peptides, and recombinant proteins
AIM V medium	GIBCO	Cat # 12055-091
DPBS w/o Ca/Mg (DPBS)	COSTAR	Cat # 21-031-CM
Clorox germicidal bleach	VWR	Cat # 8901-620
Reagent alcohol 70% (v/v)	Ricca	Cat # 3546.70–5
Molecular Biology Grade Water (MBGW)	Hyclone	Cat # SH3053802
SPRISelect	Beckman Coulter	Cat # B23319
Dynabeads™ MyOne™ SILANE	10x Genomics	Cat # PN-2000048
DNA Suspension Buffer (“Low TE”)	TEKnova	Cat # T0220
10% Tween 20 solution	Bio-Rad	Cat # 1610781
50% Glycerol	TEKnova	Cat # G1798
Buffer EB	QIAGEN	Cat # 19086
Ethanol, 200 proof	Pharmco	Cat # 111000200CSPP
Bovine Serum Albumin (BSA)	Sigma-Aldrich	Cat # A2934-100G
NxGen RNAse Inhibitor (40U/ul)	Lucigen	Cat # 30281-2
KAPA HiFi HotStart ReadyMix	Roche	Cat # 7958935001
PhiX control library	Illumina	Cat # FC-110-3001
Human TruStain FcX	BioLegend	Cat # 422302
DMSO (anhydrous)	Invitrogen	Cat # D12345
Cell Staining Buffer	BioLegend	Cat # 420201
Brilliant Stain Buffer Plus	BD Horizon	Cat # 566385
FluoroFix Buffer (contains 4% p-formaldehyde)	BioLegend	Cat # 15713
Critical commercial assays
ViaStain AOPI Staining Solution	Nexcelom Bioscience	Cat # C52-0106-25mL
BD FVS510 Live Dead Stain	BD Horizon	Cat # 564406
Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v3.1	10X Genomics	Cat # PN-1000121
Chromium Next GEM Chip G Single Cell Kit	10X Genomics	Cat # PN-1000120
HS NGS Fragment Kit (1 -6000bp)	Agilent	Cat # DNF-474-0500
Quant-iT™ PicoGreen™ dsDNA Assay Kit	Invitrogen	Cat # P7589
Oligonucleotides
HTO-cDNA Custom Primer 5′ GTG ACT GGA GTT CAG ACG TGT GCT C	Integrated DNA Technologies	N/A
SI PCR Custom Primer 5′ AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC GCT C	Integrated DNA Technologies	N/A
Single Index Kit T Set A	10X Genomics	Cat # PN-1000213
HTO i7 Sample Index Plate, custom	Integrated DNA Technologies	N/A
Software and algorithms
FlowJo	BD Biosciences	v 10.7
SpectroFlo	Cytek	v 2.2.0.4
Other
50 mL polypropylene Falcon conical tube	COSTAR	Cat # 352098
Serological pipettes (5, 25, 50mL)	VWR	Cat # 89130-896, 900, 902
Aspirating pipettes (2 mL)	VWR	Cat # 414004-265
Pipette Tips RT LTS 1000μL wide-orifice, filter	Mettler-Toledo Rainin	Cat # 30389218
Pipette Tips RT LTS 1000μL, filter	Mettler-Toledo Rainin	Cat # 30389213
Pipette Tips RT LTS 200μL, filter	Mettler-Toledo Rainin	Cat # 30389240
Pipette Tips RT LTS 20μL, filter	Mettler-Toledo Rainin	Cat # 30389226
PCR 8 -tube strips (0.2mL)	USA Scientific	Cat # 1402–4700
Eppendorf Safe-Lock tubes (1.5 mL)	Eppendorf	Cat # 022363204
Eppendorf snap cap tubes (5 mL)	Eppendorf	Cat # 0030119487
96 well 0.8 mL polypropylene deep well storage plate	Thermo Fisher Scientific	Cat # AB0765
Cellaca MX cell counter plates	Nexcelom Bioscience	Cat # CHM24-A100-020
Cellometer SD100 Cell-Counting slides	Nexcelom Bioscience	Cat # CHT4-SD100-002
Microseal B plate seal	Bio-Rad	Cat # AB0765
96-well skirted Eppendorf plate	Eppendorf	Cat # 951020401
96-well semi skirted Eppendorf plate	Eppendorf	Cat # 0030129326
5 mL polystyrene tubes	Falcon/VWR	Cat # 60819-820
300 mL Clear Advantage™ Reservoirs (Polypropylene)	Integra	Cat # 6348
Heavy Duty Aluminum 18in by 500ft	Foilman	Cat # B07BR29XV7
Griptips, Low Retention Tips in Racks, 300 μL, Green	Integra Biosciences	Cat # 6535
Pipet-Lite™ LTS Pipette L-1000XLS+	Mettler-Toledo Rainin	Cat # 17014382
Pipet-Lite™ LTS Pipette L-200XLS+	Mettler-Toledo Rainin	Cat # 17014391
Pipet-Lite™ LTS Pipette L-20XLS+	Mettler-Toledo Rainin	Cat # 17014392
Pipet-Lite™ LTS Pipette L-2XLS+	Mettler-Toledo Rainin	Cat # 17014393
Pipet-Lite™ Multi Pipette L8-200XLS+	Mettler-Toledo Rainin	Cat # 17013805
Pipet-Lite™ Multi Pipette L8-20XLS+	Mettler-Toledo Rainin	Cat # 17013803
Pipet-Lite™ Multi Pipette L8-10XLS+	Mettler-Toledo Rainin	Cat # 17013802
Beckman Avanti J-15R Centrifuge	Beckman Coulter	Cat # B99517
Beckman JS-4.750 Swinging-Bucket Rotor & Buckets	Beckman Coulter	Cat # B77580
Beckman JS-4.750 Swinging-Bucket Rotor Microplate Carriers	Beckman Coulter	Cat # B83980
Beckman centrifuge tube bucket covers	Beckman Coulter	Cat # 392805
Beckman centrifuge microplate carrier covers	Beckman Coulter	Cat # 393070
Beckman 30 mm conical tube adapter	Beckman Coulter	Cat # 393267
Eppendorf Centrifuge 5430	Eppendorf	Cat # 5430
Serological Pipette Controller	Thermo Scientific	Cat # 9511
Mini-Centrifuge	Southwest Science	Cat # SC1012
Vortex Mixer	Southwest Science	Cat # SBV1000
Cold Block for 1.5 mL tubes	Corning	Cat # 432038, 432036
Cold Block for 96-well skirted/semi-skirted plates	Integra	Cat # 6250
DynaMag-96 Side Skirted magnetic plate stand	Invitrogen	Cat # 12027
Plate Heat Sealer	4titude	Cat # 4ti-0665
Chromium Controller	10x Genomics	Cat # 1000202, 1000204
Fragment Analyzer	Agilent	Cat # M5311AA
Spectramax iD3	Molecular Devices	ID3-STD
Thermal Cycler	Bio-Rad	Cat # 1851196
Cellaca MX high-throughput automated cell counter	Nexcelom Bioscience	Cat # Cellaca MX
Cellometer Spectrum Image Cytometry System	Nexcelom Bioscience	Cat # Cellometer Spectrum
Integra VIAFLO 96 handheld electronic 96 channel pipette	Integra Biosciences	Cat # VIAFLO 96
PCR 96 well Cooling block	Integra Biosciences	Cat # 6250
Milli-Q IQ 7000	Milli-Q	Cat # ZIQ7000T0
Cytek Aurora spectral flow cytometer	Cytek Biosciences	Cat # Aurora