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GEM-Reverse Transcription Master Mix (GEM-RT MM)
| Reagent | Final concentration | Amount |
|---|---|---|
| RT Reagent B | 58.2% | 18.8 μL |
| Template Switch Oligo | 7.4% | 2.4 μL |
| Reducing Agent B | 6.2% | 2.0 μL |
| RT Enzyme C | 26.9% | 8.7 μL |
| NxGen RNAse Inhibitor (40U/μL) | 16U, 1.2% | 0.4 μL |
| Total | N/A | 32.3 μL |
Store on ice for up to 1 h.
