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. 2021 Nov 5;2(4):100900. doi: 10.1016/j.xpro.2021.100900

cDNA amplification reaction mix

Reagent Final concentration Amount
Amp Mix 75.8% 50 μL
cDNA Primers 22.7% 15 μL
HTO cDNA Additive Primer (0.2 μM) 3 nM, 1.5% 1 μL
Total N/A 66 μL

Store on ice for up to 1 h.