Figure 4.

Typical results received from the experiments

Calculation of the results as described in this protocol will give you the ECAR/OCR ratio of control cells (white boxes) and gramicidin treated, i.e., K⁺ depleted, cells (magenta boxes), and the effect of high K⁺ on this ECAR/OCR ratio (B). High ratios in (A) indicate high glycolytic vs. oxidative activity, low ratios indicate low glycolytic vs. oxidative activity. Ratios above 1 in (B) indicate a stimulatory, ratios below 1 an inhibitory effect of K⁺ on glycolytic vs. oxidative activity. The presented findings indicate for INS-1 832/13 that their metabolic activity is independent on [K⁺]_i, while HeLa and HEK293 cells show a dependency on [K⁺]_i. Boxes indicate the median and the first and third quartile. Lower and upper whiskers indicate 5-95 percentile. Part of the figure has been published in Bischof et al. (2021). Figure reuse with permission from Bischof et al. (2021).