REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides and recombinant proteins
2-Deoxy-D-glucose	Tocris	n/a
2-Mercaptoethanol	Thermo Fisher Scientific	n/a
50× MEM Amino Acids Solution	Thermo Fisher Scientific	n/a
100× MEM Vitamin Solution	Thermo Fisher Scientific	n/a
100× Sodium Pyruvate Solution	Thermo Fisher Scientific	n/a
Antimycin-A	Abcam	n/a
CaCl22H2O	Carl Roth	n/a
Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP)	Santa Cruz Biotechnology	n/a
D-Glucose H2O	Carl Roth	n/a
DMEM, high glucose, HEPES	Thermo Fisher Scientific	n/a
FeCl2	Sigma-Aldrich	n/a
Fetal Calf Serum	Thermo Fisher Scientific	n/a
Gibco RPMI 1640 Medium	Thermo Fisher Scientific	n/a
Gramicidin	Sigma-Aldrich	n/a
HEPES	Carl Roth	n/a
KCl	Carl Roth	n/a
KHCO3	Carl Roth	n/a
KH2PO4	Carl Roth	n/a
KOH	Carl Roth	n/a
L-Glutamine (200 mM)	Thermo Fisher Scientific	n/a
MgSO4	Carl Roth	n/a
NaCl	Carl Roth	n/a
NaHCO3	Carl Roth	n/a
NaH2PO4	Carl Roth	n/a
NaOH	Carl Roth	n/a
Oligomycin-A	Tocris	n/a
Penicillin-Streptomycin (100×)	Thermo Fisher Scientific	n/a
Phenol red solution	Sigma-Aldrich	n/a
Phosphate buffered saline	Thermo Fisher Scientific	n/a
Rotenone	Tocris	n/a
Trypsin-EDTA (0.5%)	Thermo Fisher Scientific	n/a
Critical commercial assays
Pierce BCA Protein Assay Kit	Thermo Fisher Scientific	n/a
Seahorse XFe96 Flux Pak	Agilent Technologies	n/a
Experimental models: cell lines
HEK293	ATCC	n/a
HeLa S3	ATCC	n/a
INS-1 832/13	Gift from C.B. Newgard (Department of Pharmacology and Cancer Biology, Duke University School of Medicine, USA)	n/a
Software and algorithms
Excel Office 365	Microsoft	n/a
Prism 8	GraphPad	n/a
Seahorse Wave Desktop Software	Agilent Technologies	n/a
Other
15 mL Centrifuge Tubes	Corning	n/a
37°C Incubator without CO2	Binder	Cat. No. B28
37°C Incubator, 5% CO2, humidified	Thermo Fisher Scientific	Cat. No. 51026331
37°C Water bath	Phoenix WB-22	n/a
Bürker-Türk cell counting chamber	Merck	Cat. No. MDH-2N1-50PK
Centrifuge	Eppendorf	Cat. No. 5702000010
Seahorse XF96 Analyzer	Agilent Technologies	n/a
Seahorse XF96 Cell Culture Microplates	Agilent Technologies	Cat. No. 101085-004
Seahorse XF96 FluxPak	Agilent Technologies	Cat No. 102416-100
Sterile workbench	Thermo Fisher Scientific	HS12