FIG. 3.
Accumulation of functional Can1p correlates with nonsense suppression of can1-100. (A) 3H-labeled arginine uptake in yeast strains with the indicated UPF/NMD and CAN1 genotypes. The control yeast strain harboring the CAN1 allele is PLY148 (36). WT, wild type. Error bars indicate standard deviations. (B) Western analysis of Can1p levels. Lysates of yeast strains with the indicated UPF/NMD genotypes and bearing either CAN1 or can1-100 plasmids were analyzed by Western blotting with HA-specific antibodies. The lower panel is a longer exposure of the same blot shown in the upper panel.