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. 2000 Jul;20(13):4591–4603. doi: 10.1128/mcb.20.13.4591-4603.2000

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Copyright © 2000, American Society for Microbiology

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FIG. 4 — can1-100 nonsense suppression is only partially attributable to increased mRNA abundance. (A) Northern analysis of can1-100 mRNA levels. RNA isolated from yeast strains with the indicated genotypes was analyzed by Northern blotting with probes specific for can1-100 mRNA and SCR1 RNA (the latter to serve as an internal loading control). Each of the indicated strains contained either a high-copy-number can1-100 plasmid (YEp can1-100), a single-copy can1-100 plasmid (YCp can1-100), or an empty vector as a control (YEp). WT, wild type. (B) can1-100 steady-state mRNA levels. Data from the blot in panel A were quantitated by phosphorimaging, standardized to SCR1 RNA levels, and normalized to data for the upf1Δ strain. (C) Canavanine sensitivities of strains harboring single-copy or high-copy-number plasmids. Suppression assays analogous to those shown in Fig. 2 were used to define the canavanine (Can.) sensitivities of cells with different UPF/NMD genotypes.

FIG. 4 — can1-100 nonsense suppression is only partially attributable to increased mRNA abundance. (A) Northern analysis of can1-100 mRNA levels. RNA isolated from yeast strains with the indicated genotypes was analyzed by Northern blotting with probes specific for can1-100 mRNA and SCR1 RNA (the latter to serve as an internal loading control). Each of the indicated strains contained either a high-copy-number can1-100 plasmid (YEp can1-100), a single-copy can1-100 plasmid (YCp can1-100), or an empty vector as a control (YEp). WT, wild type. (B) can1-100 steady-state mRNA levels. Data from the blot in panel A were quantitated by phosphorimaging, standardized to SCR1 RNA levels, and normalized to data for the upf1Δ strain. (C) Canavanine sensitivities of strains harboring single-copy or high-copy-number plasmids. Suppression assays analogous to those shown in Fig. 2 were used to define the canavanine (Can.) sensitivities of cells with different UPF/NMD genotypes.