FIG. 6.

Overexpression of UPF1 in upf/nmd mutant strains does not affect can1-100 mRNA abundance. (A) Northern analysis of can1-100 mRNA levels. Total RNA isolated from yeast strains with the indicated genotypes was analyzed by Northern blotting as described in the legend to Fig. 4. Each of the mutant strains contained either a high-copy-number UPF1 plasmid (YEp-UPF1) or an empty vector as a control (YEp). (B) Quantitation of can1-100 steady-state mRNA levels. can1-100 mRNA levels were determined, standardized to SCR1 RNA, and normalized to data for the upf1Δ strain as described in the legend to Fig. 4.