TABLE 2.
Overexpression of UPF1, NMD2, or UPF3 in wild-type and upf/nmd strainsa
| Strain | Canavanine sensitivity (μg/ml)
|
|||
|---|---|---|---|---|
| YEp | YEp-UPF1 | YEp-NMD2 | YEp-UPF3 | |
| Wild type | 300 | 300 | 300 | 300 |
| upf1Δ | 25 | 300 | 25 | 25 |
| nmd2Δ | 35 | 75 | 300 | 35 |
| upf3Δ | 35–40 | 75 | 25 | 300 |
| upf1Δ nmd2Δ | 25 | 75 | 25 | 25 |
| upf1Δ upf3Δ | 25 | 75 | 25 | 25 |
| nmd2Δ upf3Δ | 35 | 75 | 35 | 35 |
| upf1Δ nmd2Δ upf3Δ | 50 | 125 | 50 | 50 |
a
Suppression assays are summarized, with the concentration of canavanine at which each strain began to exhibit the canavanine-sensitive phenotype indicated (canavanine sensitivity). Yeast strains of the indicated genotypes were transformed with the high-copy-number vector alone (YEp; control) or high-copy-number plasmids expressing UPF1, NMD2, and UPF3 (YEp-UPF1, YEp-NMD2, and YEp-UPF3, respectively).