Fig. 3.

Hapln1 and Sp6 gene promoters are transactivated by Sp6. A. Transfection experiment in HEK-293 cells were performed with luciferase reporter constructs for the promoterless pGL3 vector, 0.9 Kb promoter (pHapln1), a promoter with a 4 bp substitution (pHapln1-4bpSub) in the core AT-rich Sp6 binding sequence and with pSp6–53 bp, a construct containing 53bp of the rat Sp6 gene containing Sp6 target sequence. In these experiments, no addition, pCMV empty vector, pCMV-Sp6 and pCMV-Sp6-Vp16 expression vectors were utilized and following transfection, luciferase reporter activity was assessed. B. As described in the Material and Methods, transient transfection experiments were performed using pGL4.23 (a basal promoter luciferase reporter vector) and corresponding 4X wild type and mutant 4X HLBS reporter constructs.