FIGURE 2.

Functional characterization of SCN1B missense variants. (A–C) Normalized conductance-voltage (G-V; open circles) and channel availability relationship (I-V; filled circles) of β1^WT (black), β1^R46C (red), β1^R60C (blue), or β1^{V158 M} (green) coexpressed with human (h) Na_V1.1, Na_V1.2 or Na_V1.6. G-V and I-V V_1/2 values are reported in Table S3. (D–F) Normalized recovery from fast inactivation using the same color scheme measured over a 40-ms timeframe using a double-pulse protocol to the maximum current of the I-V in A–C. Half-life recovery time points (ms) are presented in Table S3. Error bars are SEM with n = 5–11; *p < .05, **p < .01, ^#p < .001. (G–I) Rate (τ) of channel fast inactivation. Half-life time points (ms) are presented in Table S3. Error bars reflect SEM with n = 5–6; *p < .05, **p < .01, ^#p < .001