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. 2021 Oct 29;12:658197. doi: 10.3389/fphar.2021.658197

FIGURE 4.

FIGURE 4

CUDC-907 inhibits proliferation, causes G2/M arrest, and induces apoptosis in HCC cells. (A) Cell apoptosis detected by flow cytometry. HuH-7 and SMMC-7721 cells were treated with 0, 5, 15, 30, and 60 nM CUDC-907 for 36 h. (B) Cell cycle changes detected by flow cytometry. HuH-7 and SMMC-7721 cells were treated with 0, 5, 15, 30, and 60 nM CUDC-907 for 24 h. The statistic is referring to G2 cell phase. (C) Colony-formation assays. HuH-7 and SMMC-7721 cells were measured with crystal violet staining after treatment with CUDC-907 at 0.625–5 nM for 14 days. (D) HuH-7 and SMMC-7721 cells were collected for western blotting with the indicated antibodies after treatment with CUDC-907 for 24 h or 36 h. *p < 0.05, **p < 0.001. (C-PARP and Cleaved caspase-3 were compared with PARP or caspase-3 of NC group; P21 was compared with that of 60 nM group; other results were compared with NC group).