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. 2021 Oct 29;12:658197. doi: 10.3389/fphar.2021.658197

FIGURE 5.

FIGURE 5

Western blotting of downstream regulated proteins of CUDC-907 and immunohistochemistry staining. (A) The expression of c-Myc is regulated by the HDAC and PI3K/AKT/mTOR pathway, and both can be inhibited by CUDC-907. (B) Expression levels of proteins [c-Myc, p-AKT, p-S6, p-4EBP1, and acetyl histone H3K9 (H3K9ac)] in HCC cell lines affected by CUDC-907 were detected by western blotting. GAPDH was set as the internal reference. (C) Expression levels of proteins (same as figure 5B) from xenografted tumor with or without CUDC-907 treatment were detected. (D) Immunohistochemistry staining. Representative images of Ki67, c-Myc, and TUNEL staining of xenografts treated with CUDC-907 or vehicle. (E) Quantification of immunohistochemistry staining was calculated by the immunohistochemistry score method. The percentage of apoptotic area in tumor slides of TUNEL staining was calculated by IMAGE J software. *p < 0.05, **p < 0.001 (result compared with Vehicle group).