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. 2021 Oct 29;8:746822. doi: 10.3389/fmolb.2021.746822

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Copyright © 2021 Goumenou, Delaunay and Pichon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Configuration of the serial TPPMRs for online proteolysis and glycopeptide enrichment prior to nLC-ESI-MS/MS, Setup configuration: Operational position 1: on-line digestion and glycopeptides enrichment, Valve positions: I: solid and II: dotted lines. Operational position 2: PNGase F injection and loading to trap column, Valve positions: I: dotted and II: solid lines. Operational position 3: LC/MS run of deglycosylated peptide and re-conditioning of TPPMR, Valve positions: I: solid and II: dotted lines. TPPMR: thermoresponsive porous polymer membrane reactor. nLC-ESI-MS/MS: nanoflow liquid chromatography-tandem mass spectrometry. T: Trypsin immobilized TPPMR. L: Lectin immobilized TPPMR. Reprinted with permission from Yang et al., Anal. Chem. 2018, 90, 3,124–3,131, Copyright ^© 2018, American Chemical Society.